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Flow Cells: PDMS Casting and System Integration

Brochure - Applications
In order to immobilize molecules or cells in microfluidic channels, the flow-through system should be designed such that it can be closed and opened again. It would be also ideal if one and the same fluidic system could be utilized for different analytical (e.g., electrochemical or optical) methods by very simple modification and also allow efficient cleaning.

For this purpose, provided are flow-through chambers with removable coverslips for the microscope. These flow cells are mounted in seconds and can be applied in cell imaging or single molecule fluorescence. If needed, electrical signals can be transferrd or optical fibers mounted.

Layout and assembly of the microsystem can be modified in a wide range. So in addition to PDMS, other materials can be used and microvalves and microelectrodes can be integrated. Due to the easy and modular setup, even the most complicated methods in screening and nanotechnology can be realized.

Our approach is to provide modular solutions for microstructuring that are complementary to the microfluidic applications that we work with. Provided are numerous approaches towards developing 3-dimensional surface topographies. These systems are developed for precision liquid handling for microscopic imaging and can be integrated with the Nano-Plotter platform.

Catalog

      Mechanical Components

      Code Description
      A040-018 MicCell Support, standard, aluminum
      A040-019 MicCell Support, with printed circuit board
      A040-109 MicCell Lid, 22x22 mm
      A040-110 MicCell Lid, 22x50 mm

      MicCell Fluid Processor

      Code Description
      A050-005 MicCell Fluid Processor, 4-channel version
      A050-007 MicCell Fluid Processor, standard version
      A050-008 MicCell Twin Fluid Processor
      A110-015 Smart Valve Upgrade, 4-port

      MicCell Casting Station

      Code Description
      A040-030 Silicon Master Chip, structure height 2 - 50 µm
      A040-031 Silicon Master Chip, structure height 50 - 150 µm
      A040-033 MicCell PDMS Casting Station
      A040-091 MicCell Channel Spacer

      MicCell Accessories

      Code Description
      A040-080 Hydrogel Microvalve PV5, PEEK housing
      A040-081 Hydrogel Microvalve PV6, PEEK housing
      A040-100 Hydrogel Microinjector PV6, PEEK housing
      A040-101 Hydrogel Microvalve PV6, naked chip
      A040-084 MicCell Sample Carrier

      MicCell Consumables

      Code Description
      A040-105 MicCell PDMS Channel Plate 22x22 mm, recycled
      A040-106 MicCell PDMS Channel plate 22x22 mm, new
      A040-107 MicCell PDMS Channel Plate for Sample Carrier
      A040-108 MicCell PDMS Channel Plate 22x50 mm, new
      A040-111 MicCell PDMS Channel Plate 22x50 mm, recycled
      A040-112 MicCell PDMS CP 22x22 mm with 4 electrodes
      A040-026 MicCell Glass Slide, with SiR channel
      A040-027 Coverslip, 150 µm, with Pt electrodes and SiR channel
      A040-044 Coverslip, 150 µm thick, glass, customized size
      A040-102 Coverslip, 150 µm thick, with platinum electrodes
      A040-103 Coverslip, 150 µm thick, with ITO electrodes
      A040-104 Coverslip, 150 µm, with SiO -passivated Pt electrodes
      A660-300 MicCell PDMS Syringe, 5 ml
      A660-301 MicCell PDMS Syringe, 1 ml
      A660-302 MicCell Injection Needles
      A660-303 MicCell PDMS Mixing Glass

      MicCell Software

      Code Description
      A070-089 MicCell Software, standard version

      Microcontact Printing

      Code Description
      A080-410 CAD design
      A080-400 Chromium Mask 5 inch
      A040-030 4"-Si-Master Teflon coated and cutted
      A040-600 µCP-Device
      A040-601 FLEXIPERM, PDMS-well-gasket with 8 or 12 wells


HTS Resources, LLC 325 W. Washington St #2-150, San Diego, CA 92103 USA